Journal: Molecular cell

Article Title: Balancing of mitochondrial translation through METTL8-mediated m 3 C modification of mitochondrial tRNAs

doi: 10.1016/j.molcel.2021.10.018

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Table of used oligonucleotides, related to STAR Methods Metabion N/A DNA oligonucleotide sequences for mt-tRNA in vitro transcription, see Table S1 .

Techniques: Transduction, Virus, Recombinant, Adjuvant, Plasmid Preparation, cDNA Synthesis, Western Blot, Microscopy, Northern Blot, Cloning, Mutagenesis, In Vitro, Modification, Software

Journal: Molecular cell

Article Title: Balancing of mitochondrial translation through METTL8-mediated m 3 C modification of mitochondrial tRNAs

doi: 10.1016/j.molcel.2021.10.018

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Table of used oligonucleotides, related to STAR Methods Metabion N/A DNA oligonucleotide sequences for mt-tRNA in vitro transcription, see Table S1 .

Techniques: Transduction, Virus, Recombinant, Adjuvant, Plasmid Preparation, cDNA Synthesis, Western Blot, Microscopy, Northern Blot, Cloning, Mutagenesis, In Vitro, Modification, Software

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Cotranslational folding cooperativity of contiguous domains of α-spectrin

doi: 10.1073/pnas.1909683117

Figure Lengend Snippet: Release rates and estimation of pulling forces. (A) The rate of release (kR) obtained from pulse-chase experiments (SI Appendix, Fig. S4 and Dataset S2), the fraction full-length protein (fFL) measured under standard experimental conditions (20-min incubation in PURExpress in the continuous presence of [35S]Met), and the pulling force F calculated using Eq. 1 (k0=3.0×10−4s−1, Δx‡=0.65nm). The constructs are from Kudva et al. (12) and are colored to match those in B and SI Appendix, Figs. S4 and S5. (B) F values calculated from Eq. 1 plotted against the standard fFL values, with constructs colored as in A. The least-squares fit line is indicated by the blue line, and the analytic relation Eq. 3 between F and fFL, assuming an average delay time Δt = 550 s (approximately equal to half the standard incubation time), is shown as a red curve.

Article Snippet: The PURExpress in vitro transcription–translation system was purchased from NEB.

Techniques: Pulse Chase, Incubation, Construct

Journal: International Journal of Molecular Sciences

Article Title: Evaluation of Various Alternative Economical and High Throughput SARS-CoV-2 Testing Methods within Resource-Limited Settings

doi: 10.3390/ijms232214350

Figure Lengend Snippet: Comparison of the sensitivity and specificity of the commercial SARS-CoV-2 RT-PCR assay kits.

Article Snippet: Five commercially available RT-PCR SARS-CoV-2 assay kits from different manufacturers were selected in this study for the method comparison including the Thermo Fisher TaqPathTM COVID-19 Assay Kit (Thermo Fischer Scientific, Pleasanton, CA, USA), an internationally approved kit, and the four alternative RT-PCR SARS-CoV-2 assay kits: Nucleic Acid COVID-19 Test Kit (SARS-CoV-2) (Wuhan Easy-diagnosis Biomedicine, Wuhan, China), abTES TM COVID-19 qPCR I Kit (Anatech Instrument (PTY) LTD, Meadowbrook, Business Estate, Sloane Park, Gauteng, South Africa), PCL COVID19 Speedy RT-PCR Kit (PCL Inc. Multiplex In Vitro Diagnostic Global Leader, Seoul, South Korea), and the PCLMD nCoV One-Step RT-PCR Kit (PCL Inc. Multiplex In Vitro Diagnostic Global Leader, Seoul, South Korea).

Techniques: Comparison, One Step RT-PCR